Agar Diffusion Method for the Assay of Colicins

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Agar diffusion method for the assay of colicins.

An agar diffusion method for the assay of colicins A, B, D, E(2), E(3), and K is described. The assays were performed in large, square pyrex dishes that contained an agar layer seeded with an indicator organism sensitive to the colicin. The samples were applied to the agar in steatite beads positioned in a randomized sequence. The plates were stored at 4 C for 24 hr to allow the colicins to dif...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1968

ISSN: 0003-6919

DOI: 10.1128/aem.16.10.1468-1474.1968